蛹虫草菌丝体中单核苷酸类组分的高效液相色谱分析

Determination of Flavour Nucleotides Content in Cultured cordyceps militaris(Fr.) Link mycelium by High Performance Liquid Chromatography

目的测定蛹虫草菌丝体中5-′CMP(5′-胞苷酸)、5-′UMP(5′-尿苷酸)、5-′GMP(5′-鸟苷酸)、5-′IMP(5′-嘌呤核苷酸)、5-′XMP(5′-黄嘌呤核苷酸)、5-′AMP(5′-腺苷酸)等六种核苷酸的含量。方法用反相高效液相色谱法,以SH IMAD-ZU C18(150 mm×4.6 mm I.D,5μm)为色谱柱;以缓冲盐(KH2PO4-H3PO4)-甲醇(99.5∶0.5,V/V,pH3.75)体系为流动相,在流速0.8 mL/m in,柱温26°C,检测波长260 nm,进样量20μl的条件下对样品中5′-核苷酸的含量进行了分析测定。结果蛹虫草菌丝体中上述6种核苷酸含量分别为173.0,824.9,92.9,2114.0,79.6,231.4μg/g。结论该法快速简便,重现性好,能有效的测定样品中核苷酸类成分。

Objective To determine Flavour nucleotides （ 5′-IMP, AMP, GMP, UMP, IMP, XMP） content in Cultured cordyceps militaris（Fr- ） Link mycelium. Methods Agilent 1100, Shimadzu C18 （150 mm ×4.6 mm I. D,5 μm） and a UV detector at 260nm and 0.2 mol/L KH2PO4 - H3PO4 - methanol（99.5： O. 5, V/V, pH 3.75） solution as eluent. Injection 20μl. Result. s There are 173.0, 824.9, 92.9, 2114.0, 79.6, 231.4 μg/g in the Cultured cordyceps militaris（Fr. ） Link mycelium. Conclusion The method is simple, rapid, sensitive and reproducible.