高效液相色谱-蒸发光散射检测法测定柴胡药材中柴胡皂苷a及柴胡皂苷d的含量

Determination of Saikosaponin a,d in Radix Bupleuri by HPLC-ELSD

目的 制定柴胡药材中柴胡皂苷a、d的含量测定方法。方法 用高效液相色谱-蒸发光散射检测法（HPLC-ELSD），Alltima C18色谱柱，流动相甲醇-水（80：20），速度0．8ml·min^-1；蒸发光散射检测器参数：漂移管温度40℃，气压3.5bar。结果柴胡皂苷a和柴胡皂苷d分别在1．908-19．08μg（r=0．9996）和1．804-18．04μg（r=0．9992）范围内线性关系良好，平均回收率分别为98．35％（RSD=1．26％）和97．64％（RSD=1．58％）。结论 该方法简便、灵敏、可靠、准确、重现性好，可作为柴胡药材的质量控制方法。

Objective To determine saikosaponin a, d in Radix Bupleuri by HPLC-ELSD. Methods HPLC-ELSD was used in the quantitative analysis by using Alhima CI8 chromatography column and methanol-water（ 80： 20） as a mobile phase. The flow rate of mobile phase was 0. 8 ml · min^-1. The tube temperature of the detector was 40℃. The press of pure air was 3.5bar. Results The linear ranges for saikosaponin a and d were 1. 908 - 19.08 μg （ r = 0. 999 6 ） and 1. 804 - 18.04μg （ r = 0. 999 2 ） , the average recoveries were 98.35% （ RSD = 1.26% ） and 97.64% （ RSD = 1.58% ） , respectively. Conclusion This method is easy, sensitive , reliable, accurate , reproducible and can be used as the quality control method of Radix Bupleuri.