突触体钙进入的实验条件分析及其意义

ALANYSIS ABOUT EXPERIMENTAL CONDITIONS OF45Ca UPTAKE INTO SYNAPTOSOME AND ITS IMPORTANCE

本文系统地研究了影响小鼠大脑皮层细胞突触体^（４５）Ｃａ摄取的实验条件。发现：在前４ｍｉｎ内。^（４５）Ｃａ摄取量与温育时间呈直线关系；突触体膜蛋白投入量以０．０６３～０．２５０ｍｇ为宜超过０．２５０ｍｇ则^（４５）Ｃａ摄取呈下降趋势；与无钙和正常钙（１．２０ｍｍｏｌ／Ｌ）培养液相比．低钙培养液（０．６ｍｍｏ－ｌ／Ｌ）更有利于突触体的钙摄取，且钙拮抗剂硝砒乙甲酯在低钙（０．６ｍｍｏｌ／Ｌ）培养液中对^（４５）Ｃａ进入突触体有促进作用；高钾刺激促进^（４５）Ｃａ摄取，但浓度以３０～５０ｍｍｏｌ／Ｌ为宜；溶剂二甲亚砜（ＤＭＳＯ）低浓度（０．０５％）有促进^（４５）Ｃａ摄取的作用；但乙醇和丙酮浓度在０．０５～６．２５％范围内则无明显影。上述实验结果为准确进行突触体钙摄取实验和测定药物对钙进入的影响提供了基础资料。

he experunental conditions of 45Ca uptake into synaptosome from the mice cerebral corticeswere studied.It was found that during the firot 4 minutes there was a straight relationshipbetween amount of 45Ca uptake and incubation tune. The range of suitable membrane protein ofsynaptosame used was between 0.063～0.250 mg and 45Ca uptake would decline when membraneprotein was more than 0.250mg Low calcium medium(0.6mmol/L)was more beneficial to the 45Cauptake than zero or normal calcium(12mmol/L)medium,and nitrendipine had a promoting effecton 45Ca uptake in the former medium. The rang of kalium concentrations for the best stunulationof 45Ca uptake was grom 30 to 50 mmol/L. DMSO had a potential effect on 45Ca uptake at theconcentration of 0.05%,but ethanol and acetone failed to show an influence on it at theconcentrations of 0.05～6.25%。All results above provide essential data for correctly carrying outexperunents of 45Ca uptake into synaptcoolne and determining the effects of drugs on 45Ca uptake.