医院消毒液细菌和HBsAg污染调查

INVESTIGATION ON BACTERIAL AND HBSAG CONTAMINATION OF COMMOND DISINFECTANT SOLUTION IN HOSPITAL

用《消毒技术规范》的细菌培养方法和乙肝表面抗原测定法（ＥＬＩＳＡ、ＳＰＲＩＡ）．对医院常用１０种消毒液共３３２份样品进行了污染调查，结果为：细菌总超标率为１１．７％．ＨＢｓＡｇ总检出率为５．８３％；细菌污染以０．１％洗消粉最严重（超标率５０％）．其次为７５％乙醇（１１．７％），１％碘伏（８．３％），２．５％碘酒（１０．８％及器械液（４．７％）；而消毒液ＨＢｓＡｇ污染以７５％乙醇为最高（１２．９％），其次为器械液（８．１％），这与两种消毒剂不能杀灭乙肝病毒结果一致。戊二醛类消毒液如“一消净”等没有细菌和ＨＢｓＡｇ污染。结果提示加强消毒产品管理，使用高效稳定的消毒液代替低质的消毒剂，对于降低消毒液污染，减少侵入性器械带菌、控制院内感染具有重要意义。

he bacterial and HBsAg contamination in 332 samples collected from ten kindsofdisinfectants had been investigated by the methods of bacterial culture and ELISA or SPRIAsccording to Disinfectant Technique Standard(1992).It was indi cated that total rate of bacterialcontamination beyond pemissible limit was 11.7%(total bacterial count excewting 250 CFU per mlwas considered as beyond permissble lunit)and positive rate ofr HB sAg was 5.83%。These results showed that the serious contamination of bacteriuin was found in 0.1%washingdisinfectant pawder,which w as 50%beyond pernilssible limit.The following were 75% ethylalcohol,2.5% tinctura iodi,1%povidine-iodine and dismfectant solution of instruments(including0.1%bromogeramine),their rates of contamination beyond permissible limit were11.7%,10.8%,8.3%and4.7%respectively。The HBsAg positive rate of 75%ethyl alcohol(13.9%)was higher than the disinfectant solution of instruments(8.1%).The others,such as“YI XIAOJIN”,2% glutaraldegyde。'Swashes' and 'Gold star' were not contaminated by bacterium andHBsAg。Above results suggest that it is important to manage and choose hospital disinfectants foraontrolling nosocomical infection.