摘要
运用荧光光谱、紫外光谱法研究了中药黄藤素与牛血清白蛋白(BSA)的相互作用。实验结果表明,黄藤素与BSA形成基态复合物导致BSA内源荧光猝灭,猝灭机理主要为静态猝灭和非辐射能量转移。测定该反应的表观结合常数KA为1.650×105L.mol-1、结合位点数n为1.502;根据F rster能量转移理论,测得供体与受体间结合距离r为2.801 nm和能量转移效率E为0.190。
The interaction between palmatine and bovine serum albumin(BSA) was investigated using fluorescence and UV/Vis spectrophotometry. The experimental results showed that the fluorescence quenching of BSA by palmatine was due to the formation of palmatine - BSA complex. The quenching mechanism was mainly attributed to static quenching and nonradiative energy transferring. The apparent binding constant KA for the reaction was 1. 650 × 10^5 L·mol^-1 and the binding site number n was 1. 502. According to the energy transfer theory of Foerster, the binding distance r and the energy-transfer efficiency E between palmatine and BSA were found to be 2. 801 nm and 0. 190, respectively.
出处
《分析测试学报》
CAS
CSCD
北大核心
2006年第5期48-51,共4页
Journal of Instrumental Analysis
基金
江苏省滩涂生物资源与环境保护重点建设实验室资助项目(JLCBE05032)
关键词
黄藤素
牛血清白蛋白
荧光猝灭
能量转移
Palmatine
Bovine serum albumin
Fluorescence quenching
Energy transfer