应用荧光定量聚合酶链反应产前诊断巨细胞病毒感染

Fluorescence quentitative PCR and its application in prenatal diagnosis of fetal cytomegalovirus infection

目的探讨FQ-PCR产前诊断巨细胞病毒感染的临床应用价值。方法用FQ-PCR对20例巨细胞病毒感染孕妇的胎儿进行产前诊断,同时对孕妇感染CMV-DNA拷贝数的高低与发生垂直传播的关系进行了探讨。结果4例胎儿绒毛组织CMV-DNA阳性,阳性率为20%(4/20),CMV-DNA平均为5.0×106,±2.2×106拷贝/ml:16羊水标本阴性,CMV-DNA<25拷贝/ml,新生儿血、尿液CMV-DNA均阴性,产后临床追踪观察3年,无CMV感染迹象。孕妇感染CMV-DNA拷贝数的几何均数为8.0251±0.3986时,有可能发生垂直传播(t=16.24,P<0.01)。结论FQ-PCR产前诊断巨细胞病毒感染结果可靠,有一定的临床应用价值。

Objective： To evuluate FQ -PCR on detection eytomegalovirus in prenatal diagnosis. Methods： Fetal diagnosis was made by fluorescence quentitative PCR. For amplification of CMV-DNA with CVS （n =4） and amniotic fluid （n = 16）. Results： The positive rate of CMV - DNA was 20% （4/20） by FQ -PCR. The average copies of CMV - DNA was 5.0×10^6±2. 2×10^6 eopies/ml. The CMV - DNA of aminiotie fluid, blood and urine of infant was negative. The copies of negative cases were 〈 25 copies/ml. The geometric mean of CMV - DNA in prenagnat women with cytomegalovirus infection was 8. 0251±0. 3986 that could be associated with vertical transmission. Conclusion： FQ - PCR is a valuabl method for detection cytomegalovirus in prenatal diagnosis.