Prostein_(31-39)特异的、HLA-A*2.1限制性NKT细胞表型及功能初步研究

Generation and characterization of HLA-A*2.1 restricted and Prostein_(31-39) specific NKT cell lines

目的诱导培养前列腺癌相关抗原Prostein特异性NKT细胞,并对其表型及功能作初步研究鉴定。方法以Prostein31-39肽、IL-2、IL-12反复刺激培养HLA-A*2.1+健康志愿者外周血单个核细胞(PBMCs),对得到的细胞系进行细胞毒功能试验,并以多色流式细胞检测技术进行表型分析。结果Prostein31-39肽、IL-2、IL-12可以诱导PBMCs产生肽特异性CD3+/CD56+NKT细胞,具有较强的肽特异性细胞毒活性及NK样细胞毒活性。结论Prostein31-39肽、IL-2及IL-12可以协同诱导培养肽特异性NKT细胞,这为研究抗原特异性NKT细胞在前列腺癌免疫治疗中的作用奠定了基础。

Objective To generate and characterize the Prostein-specific NKT cell lines. Methods Prostein31.39 peptide, IL-2 and IL-12 were used to stimulate peripheral blood mononuclear ceils of healthy male donors. 51Cr release assay and flow cytometric analysis were applied to study the cytotoxicity and phenotypic properties of the induced cell lines. Results The ex vivo expanded cell lines contained some peptide specific CD3^＋/CD56^＋ natural killer T cells, and possessed enhanced cytotoxicity function against Prostein31-39 bearing target cells and also NK-sensitive K562 cells. Conclusion IL-12 is essential for the generation of Prostein31-39 specific NKT cell lines, paving the way to further explore the possibility of using prostein specific NKT cells to overcome the immune evasion of prostate cancer cells.