重组人促红细胞生成素对大鼠视网膜缺血再灌注损伤的保护作用

Protective effect of recombinant human erythropoietin on pressure-induced retinal ichemia/reperfusion injury in rats

目的已证实重组人促红细胞生成素(recombinant human erythropoietin, rhEPO)对中枢神经细胞有抗凋亡作用。本实验探讨rhEPO对压力诱导的视网膜缺血再灌注的影响。方法大鼠经前房灌注维持眼压102 mmHg(1 mmHg=0.133 kPa),60 min,建立大鼠视网膜缺血再灌注模型。随后立即向右眼玻璃体腔注射5μl rhEPO,左眼玻璃体腔注射同等量的生理盐水,利用荧光金(fluorogold,FG)逆行示踪技术,分别于再灌注损伤后1、4、7、14 d行视网膜节细胞(retinal gan-glion cells,RGCs)计数。结果 rhEPO玻璃体腔注射后各时相点视网膜节细胞数明显高于对照组(P<0.05)。结论玻璃体腔内注射rhEPO有助于防止视网膜缺血再灌注后视网膜神经节细胞的凋亡。

Objective To evaluate the effect of recombinant human erythropoietin （rhEPO） on pressure induced retinal ischemic injury in rats. Methods The fluorogold （FG） tracing technique was used to observe the survival rate of the retinal ganglion cells （RGCs）. Retinal ischemia was induced by increasing the intraocular pressure to 102 mmHg for 60 min in 20 Long Evans rats, then 5 ml rhEPO was injected into the right vitreous chamber immediately and normal saline into the left vitreous chamber as vehicle controls. Another 5 rats without any treatment served as normal controls. All animals were sacrificed at 1,4, 7 or 14 d after reperfusion and RGCs were counted to assess the effect of rhEPO. Results The RGCs in eyes treated with intravitreal rhEPO were significantly higher than those in vehicle controls （P 〈 0.05）. Conclusion Intravitreal administration of rhEPO can ameliorate retinal injury caused by ischemia/reperfusion and may be useful to prevent neuron apoptosis in the inner retina.