脑脊液寡克隆区带检测在神经系统疾病诊断中的意义——附136例报告

A study of cerebral spinal fluid oligoclonal bands in neurological diseases.

目的探讨检测脑脊液寡克隆区带(oligoclonalbands,OCB)在神经系统疾病,尤其是多发性硬化诊断中的临床意义。方法136例神经系统疾病患者分为4组,其中多发性硬化患者32例为多发性硬化组,余下患者分为中枢神经系统感染性疾病组(42例),中枢神经系统非感染性疾病组(40例),外周神经系统疾病组(22例)。应用等电聚焦加抗生物素-生物素-过氧化酶复合物技术检测136例神经系统疾病患者脑脊液OCB,并进行对比分析。结果①多发性硬化组OCB阳性率为37%,中枢神经系统感染组为24%,中枢神经系统非感染性疾病组为5%,外周神经系统疾病组为0;多发性硬化组的OCB阳性率比其他各组高,但低于国外报道的阳性率;②多发性硬化患者中传统型多发性硬化组OCB阳性率为56%,高于视神经脊髓炎组的11%。结论中国多发性硬化患者OCB阳性率不高,提示有必要寻找更加敏感的诊断指标。

Objective： To evaluate the clinical significance of cerebral spinal fluid oligoclonal hands （OCB） in neurological diseases, especially in multiple sclerosis （MS）. Methods： 136 neurological diseases patients were divided into 4 groups, 32 cases in multiple sclerosis group, 42 cases in central nervous system infectious diseases group, 40 cases in central nervous system non-infectious diseases group, 22 cases in peripheral nervous system diseases group. Cerebral spinal fluid OCB was analyzed in 136 patients with different neurological diseases using isoelectric focusing and avldin biotln-peroxidase complex technique. The results were compared among 4 groups. Results： ①OCB positive rate was 37% in MS patients, which was lower than other reports, 24% in central nervous system infectious diseases group, 5% in central nervous system non-infectious diseases group and 0 in peripheral nervous system diseases group, respeetively.②OCB positive rate in conventional MS was 56% , which was higher than that in neuromyelitis optica. Conclusion： OCB positive rate is low in MS patients in China. It is necessary to find more sensitive Cerebral spinal fluid indicators for the diagnosis.