摘要
本研究涉及应用Idogn法对神经生长因子（NGF）进行^125I-NGF标记，并用同位素示踪法与电泳法相结合的方法研究^125I-NGF在小鼠体内的血药浓度-时间过程。结果显示，静脉注射^125I-NGF在小鼠体内的代谢规律符合二房室开放模型。分布相半衰期为0．13h，消除相半衰期为3．68h，^125I-NGF在小鼠体内分布和消除均较快，清除率为0．125L·h^-1·kg^-1，表观分布容积为0．697L·kg^-1，曲线所匍面积为16．01μg·h^-1·L^-1。
The study concerns in the label of nerve growth factor（NGF） by ^125I with Idogn method. Course of time - ^125I-NGF concentration in plasma of mouse was investigated with the method of sodium dodeeylsulfate-polyaeerylamide gel eleetrophoresis （SDS-PAGE） after iv ^125I-NGF. Result demonstrates the curve of time- ^125I-NGF concentration in plasma after iv fit a two compartment open model. Distribution phase half life is 0.13 h, elimination phase half life is 3.68 h, the mean plasma clearance is 0.125 L·h^-1·kg^-1, apparent volume of distribution is 0.697 L ·kg^-1, and the area under curve is 16.01μg·h^-1·L^-1.
出处
《原子能科学技术》
EI
CAS
CSCD
北大核心
2006年第B09期176-178,共3页
Atomic Energy Science and Technology
