RhoA亚家族与胃癌细胞恶性表型的关系

The Relationship between RhoA Subfamily Molecules and Malignant Phenotype of Gastric Cancer Cells

目的:研究RhoA亚家族成员RhoA、RhoB和RhoC对胃癌细胞恶性表型的影响。方法:系用脂质体介导法分别将编码正显性RhoA突变体(V14RhoA)与野生型RhoB(wt-RhoB)和RhoC(wt-RhoC)的真核表达载体转染入胃癌SGC7901细胞中,筛选出稳定抗性克隆,并检测转染细胞的生物学行为的变化。结果:RhoA活性增强可促进胃癌细胞的增殖,而降低对化疗药物的敏感性;上调RhoB表达抑制胃癌细胞的增殖,增强对化疗药物的敏感性;上调表达RhoC则对胃癌细胞的增殖及对化疗药物的敏感性无明显的影响,但可明显促进胃癌细胞的迁移。结论:RhoA亚家族分子参与了胃癌生长、耐药及转移等多个方面,其家族中不同分子作用不同。

Objective： To explore the effect of RhoA subfamily members, i.e., RhoA, RhoB and RhoC, on the malignant phenotype of gastric cancer cells. Methods： The vectors of pCEFL-GST/ V14RhoA, pCEFL-GST/wt-RhoB and pCEFL-GST/wt-RhoC were transfected into human gastric cancer cell SGC7901 by lipofectamine2000, respectively. Then the malignant biological behaviors of the transfected cells were explored. Results： The enhancement of RhoA activity can promote the proliferation of gastric cancer cell and can reduce the sensitivity of anticancer drugs; Up-regulation of RhoB can restrain the growth rate of gastric cancer cell and can enhance the sensitivity of anticancer drugs. The up-regulation of RhoC did not affect the proliferation and sensitivity of the anticancer drugs, but can promote the migration of gastric cancer cells. Conclusion： RhoA subfamily members can play an important but different role in regulating malignant behaviors of gastric cancer cells.