p27-CyclinD1/CDK4-Rb蛋白在不同类型甲状腺肿瘤组织中的表达及临床意义

Clinical Significance and Expression of p27,CyclinD1,CDK4-Rb in Different Types of Thyroid Tumorous Tissues

目的:探讨细胞周期调控基因蛋白p27、CyclinD1、CDK4、Rb在不同类型甲状腺肿瘤组织中的变化,为甲状腺肿瘤的发生学以及临床诊断提供参考。方法:应用流式细胞术和免疫荧光技术对甲状腺乳头状癌(40例)、甲状腺腺瘤(16例)、结节性甲状腺肿(21例)、正常甲状腺组织(10例)的CyclinD1、CDK4、p27、Rb基因蛋白的表达进行定量检测。结果:CyclinD1和CDK4基因蛋白在甲状腺乳头状癌组织中的表达量显著高于其它三组(P<0.01),而后三组间差异无显著性(P>0.05);p27基因蛋白在结节性甲状腺肿、甲状腺腺瘤和甲状腺乳头状癌表达量依次减少,均低于正常甲状腺组织(P<0.05);Rb基因蛋白的表达量在正常甲状腺组织、甲状腺腺瘤、结节性甲状腺肿、甲状腺乳头状癌中依次减少,但各组间无显著性差异(P>0.05)。结论:CyclinD1/CDK4高表达、p27低表达在甲状腺乳头状癌的发生中起着重要的作用,而Rb基因在甲状腺乳头状癌的发生中可能不起主要作用。

Objective： To investigate the expressions of cell cycle controlling genes, such as p27, CyclinD1, CDK4 and Rb in different types of thyroid tumorous tissues, in order to offer the data for carcinogenesis of the thyroid and information for clinical diagnosis. Methods： Flow cytometry and Immunofluorescence technique were used to perform the quantitative detection for the expression of p27, CyclinD1, CDK4 and Rb proteins in 40 cases With papillary carcinoma of the thyroid （PTC）, 16 with thyroid adenoma （TA）, 21 with nodular goiter （NG） and 10 with normal thyroid （NT）. Results： The upregulated expression of Cyclin D1, and CDK4 in PTC tissues was significantly higher than in other three groups （P〈0.01）, and no difference was found in NT, NG and TA tissues （P〉0.05）. The expression of p27 was down-regulated in NG, TA and PTC groups comparing to the NT group （P〈0.05）. There was no significant difference among PTC, TA and NG tissues （P〉0.05）. However, the difference between the expression of Rb and other four types of thyroid tumour tissues was not significant （P〉0.05）. Conclusions： The over-expressions of CyclinD1 and CDK4 and the lower expression of p27 play important roles in carcinogenesis of PTC, whereas, Rb does little effect probably.