脂蛋白脂酶Ser447Ter基因多态性与动脉硬化性脑梗死的相关研究

Associations between Ser447Ter gene polymorphism of lipoprotein lipase and atherosclerotic cerebral infarction

目的研究脂蛋白脂酶(lipoprotein lipase ,LPL)Ser447Ter基因多态性与动脉粥样硬化性脑梗死(atherosclerotic cerebral infarction,CI)发病的关系及其对血脂水平、颈动脉斑块的影响。方法对166例CI患者及72名健康成人采用聚合酶链反应-限制性片段长度多态性方法检LPLSer447Ter基因多态性,颈动脉超声多普勒检查颈总动脉内膜中层厚度(intima-media thickness ,IMT)和颈动脉斑块(carotid artery plaque ,CAP)的形状及大小。结果 CI组CG+GG基因型甘油三酯(triglyceride ,TG)含量比CC基因型明显降低(P=0 .001) ,高密度脂蛋白胆固醇(high densitylipoprotein cholesterol ,HDL-C)含量明显增高(P=0 .007) ;对照组CG+GG基因型TG含量比CC基因型低(P=0 .041) ;CI组G等位基因频率低于对照组(P=0 .014) ;LPL Ser447Ter基因多态性与颈总动脉IMT和CAP分级无明显相关。结论 LPLSer447Ter基因多态性与血脂变化及脑梗死的关系密切,G等位基因可能引起血TG降低、HDL-C升高;G等位基因可能是脑梗死的保护基因型。

Objective To investigate the relationship between lipoprotein lipase （LPL） gene Ser447Ter polymorphism and atherosclerotic cerebral infarction （CI）, and to investigate the effect of Ser447Ter polymorphism on plasma lipids, carotid intima-media thickness （IMT） and carotid artery plaque （CAP） in patients with CI. Methods PCR-restrction fragment lengh polymorphism （PCR-RFLP） technique was used to detect LPL gene Ser447Ter polymorphism in 166 patients with CI （CI group） and 72 healthy subjects （control group）. Carotid IMT and CAP were measured with carotid color ultrasonographic doppler for the patients and the controls. Results CG ＋ GG carriers had lower plasma triglyceride （TG） levels and higher high density lipoprotein cholesterol （HDL-C） levels than CC carriers in CI group （P = 0.001 and P = 0.007 respectively）. CG＋ GG carriers had lower plasma TG levels than CC carriers in control group , P = 0.041 ）. The frequency of G allele in CI patients was significantly lower than that in control subjects （ P = 0.014）. There was no statistical correlation between LPL Ser447Ter gene polymorphism and carotid IMT and CAP. Conclusion The Ser447Ter polymorphism of LPL gene is significantly associated with plasma lipids and CI. G allele genotype may lead to decrease of plasma TG and increase of plasma HDL-C. G allele may he a protective genotype of CI.