玉米成熟胚和茎尖愈伤诱导及其植株再生能力比较

Comparison between Maize Mature Embryo and Shoot Tip Callus Induced and Plant Regeneration

以3种不同基因型的成熟胚和茎尖两种不同外植体为材料,对它们的愈伤组织诱导,继代培养和分化进行了研究。结果表明:玉米的成熟胚和茎尖愈伤诱导率相关,但两种外植体诱导出的愈伤组织状态明显不同,茎尖诱导出的愈伤组织状态普遍比成熟胚的好;两种外植体的愈伤分化率差异明显,成熟胚愈伤组织最高分化率为2.13%,茎尖愈伤组织最高分化率可达21.2%。

This paper studies the initiation, maintenance and differentiation of callus from mature embryo and shoot tip of three genotypes in corn. The results showed that maize mature embryo and the callus induced frequency were correlated between mature embryo and shoot tip, but the callus quality was obviously different. Callus obtained from shoot tip were generally better than that of mature embryo. The differentiation frequency had great difference between these two kinds of callus, only 2.13% differentiation frequency could be obtained on mature embryo callus, and the differentiation frequency on shoot tip callus could be up to 21.2%.