应激与非应激状态下急性酒精中毒大鼠洗胃效果的研究

A survey of gastric lavage for acute alcoholism rats under stress and non-stress state and its effect

[目的]研究洗胃对应激与非应激状态下急性酒精中毒大鼠胃黏膜的影响。[方法]采用析因实验设计,将120只Wistar大鼠分为应激后酒精中毒不洗胃组、应激后酒精中毒洗胃组、酒精中毒不洗胃组、酒精中毒洗胃组、空白对照组,复制应激与非应激酒精中毒模型,给应激后酒精中毒洗胃组和酒精中毒洗胃组大鼠洗胃,直至洗出液用Vitali反应检查法检测洗出液呈无色为止。各组分别于酒精中毒后2h、24h、48h处死8只大鼠,制备胃黏膜大体及光镜标本,并观察胃黏膜的病理变化,进行胃黏膜损伤评分(LS)。[结果]在排除时间因素的影响时,应激后酒精中毒洗胃组与应激后酒精中毒不洗胃组LS差异有统计学意义(P<0.05);酒精中毒不洗胃组与酒精中毒洗胃组LS差异有统计学意义(P<0.05)。在排除组别因素的影响时,不同时间点中任何两个时间点LS差异均有统计学意义(P<0.05)。分组因素和时间因素有交互作用。[结论]应激状态下急性酒精中毒后清水洗胃会加重胃黏膜的损伤,不主张洗胃;而非应激状态下急性酒精中毒后,清水洗胃会加快胃黏膜的修复,提倡洗胃。消极的心理因素与疾病的发生、发展密切相关。

Objective： To investigate the influence of gastric lavage on acute alcoholism rats in stress and non - stress situation. Method： By adopting factorial experiment design, a total of 120 Wistar rats were devided into post- stress alcoholism without gastric lavage group, post- stress alcoholism gastric lavage group, alcoholism without gastric lavage group, alcoholism gastric lavage group and control group. Both stress alcoholism and non - stress alcoholism rat models were prepared. Then gastric lavage was performed for the rats in post - stress alcoholism group and alcoholism group until the fluid of lavage become clear through repeated Vitali reaction detection. Eight rats were killed at 2 hours, 24 hrs and 48 hrs after alcoholism respectively. Both rnacrospecimen and light microscopic specimen of gastric mucosa were prepared and pathological changes of gastric mucosa were observed. And gastric mucosa lesion scorings were performed. Result： There was a significant difference in lesion score （LS） between post - stress alcoholism lavage group and post - stress alcoholism non-lavage group bared on excluding of time factor influence （P 〈 0.05）. There was a significant difference in terms of LS between alcoholism non- lavage group and alcoholism lavage group （P 〈 0.05）. Based on excluding of influence of different groups, there were significant differences in terms of LS between any two groups at different time points （P 〈 0.05）, The grouping factors interacted with time factors, Conclusion： It suggested that under stress state gastric lavage with clear water for alcoholism rats may cause the injury of gastric mucosa. So gastric lavage is not recommended. While under non - stress state gastric lavage with clear water for acute alcoholism rats could promote the recovery of gastric mucosa, which was strongly advocated. Negative psychological factors are closely correlated with the occurrence and development of the disease.