补阳还五方对冠脉结扎犬心肌梗死的保护作用

On the protective action of Buyanghuanwu prescription on myocardial infarction induced by ligating coronary artery in anesthetized dog

目的:观察补阳还五方对急性心肌梗死的保护作用,比较其醇提物与水提物的作用差异。方法:将麻醉犬冠状动脉结扎造成心肌梗死模型,设补阳还五方醇提物低、高剂量组(20、40 g/kg),补阳还五方水提物低、高剂量组(20、40 g/kg),假手术组及模型对照组,以心电图ST段、血清LDH、CK、SOD和MDA、心肌梗死范围为评价指标。结果:补阳还五方醇提物及水提物均可对抗心电图ST段上移,降低血清LDH、CK及MDA,升高SOD,减少心肌梗死面积,但与原药材相当的醇提物活性明显强于水提物(P<0.01)。结论:补阳还五方对犬冠脉结扎造成的急性心肌梗死有保护作用,其醇提物优于水提物。

Objective： To observe protective action of Buyanghuanwu prescription（BP） on acute myocardial infarction and to compare the different effects between its alcohol extract（AE） and water extract （WE） . Methods： Myocardial infarction model was made by ligating coronary artery in anesthetized dog, the experiment was consistent of sham operation group, myocardial infarction group, low and high dose groups of alcohol extract （20、40 g/kg respectively）, and low and high dose groups of water extract （20、40 g/kg respectively ） . The results were evaluated by serum levels of LDH,CK,SOD ,MDA, the STsegment elevation in ECG and myocardial infarct size. Results： AE and WE could both prevent ST-segment shifting, reduce serum levels of LDH ,CK,MDA, increase serum SOD and decrease myocardial infarction area, but at the same dose of crude drugs , AE was better than WE in effect. Conclusion： BP has protective effect on myocardial infarction caused by ligating coronary artery in anesthetized dog, its AE is superior to WE in effect.