大鼠小肠对葛根素吸收的动力学研究

The absorption of puerarin in rats' intestines

采用在大鼠小肠内吸收药物的方法,对葛根素的肠吸收动力学进行了研究。利用UV双波长等吸收消去法测定小肠循环液中葛根素的含量,结果表明,对于质量浓度为50、100和200μg/mL的葛根素,在小肠内循环4 h后相应的药物吸收速率常数(Ka)分别为0.021、0.028和0.020 h-1,吸收率(Pa)分别为19.28%、21.78%和16.98%,在不同浓度下葛根素的吸收速率常数无显著差异(显著性水平因子P>0.05),该药物在肠道内的吸收呈现一级吸收动力学过程,其吸收机制为被动扩散。

The absorption kinetics of puerarin in rat intestines was studied. Puerarin solutions were circulated for 4 hours in the rat＇s small intestine and the puerarin concentration subsequently determined by dual-）, UV. At different concentrations of puerarin （50, 100,200 btg/mL）, the intestinal absorption rate constants （Ka） were 0. 021 h^- 1, 0. 030 h^- 1 and 0. 020 h^-1, and absorption percentages were 19.28 %, 21.78 % and 16.98 %, respectively. These in situ intestinal absorption experiments showed that the absorption of puerarin in rats was a firstorder process and the mechanism of absorption was passive diffusion transport.