6-氨基-7-氟-2H-1,4-苯并噁嗪-3(4H)-酮的高效液相色谱法测定

Determination of 6-amino-7-fluoro-2H-1,4-benzoxazin-3(4H)-one by HPLC

叙述了采用反向高效液相色谱法,以甲醇为溶剂,甲醇与水(体积比65∶35)为流动相,ODS为填料和紫外检测器分离测定6-氨基-7-氟-2H-1,4-苯并嗪-3(4H)-酮。该方法的标准偏差为0.29,变异系数为0.32%,回收率为99.85%,线性关系系数为0.9997。

It was introduced that quantitative analysis of 6-amino-7-fluoro -2H-1,4-benzoxazin-3（4H）-one by reversed phase HPLC, using methanol as solvent, methanol-water（65：35, by vol）as mobile phase, packing by ODS and using UV detector. The standard deviation was 0.29, coefficient of variation was 0.32%, average recovery was 99.85%, linear correlation was 0.9997.