新型高抗草甘膦N-乙酰转移酶基因的分离及其在大肠杆菌中的表达

Isolation of a novel glyphosate tolerance N-acetyltransferase gene and expression in E. coli

利用草甘膦极端污染土壤总DNA,构建了元基因组文库,筛选到一个高抗草甘膦的N-乙酰转移酶（N-acetyltransferase,GAT）基因,并利用原核表达系统对该基因进行了功能鉴定,发现其在大肠杆菌BL21中能耐受高达300 mM的草甘膦.研究结果为进行N-乙酰化途径高抗草甘膦机理研究和新型高抗草甘膦转基因作物的开发提供了理论基础.

A metagenomic library was constructed using DNA from glyphosate polluted soils, and a novel glyphosate tolerance N-aeetyhransferase（GAT） gene was screened. The gat gene＇s function analysis was performed by using the E. coli expression system, and the result indicated that BI21 which contains gat can tolerate up to 300mM glyphosate. It provided an important theoretical basis for developing glyphosate resistance transgene crops which are different from EPSPS （5- enolpyruvylshikimate-3-phosphate synthase） ones.