红豇豆根缘细胞对铝胁迫的响应

Response of root border cell in red cowpea to aluminum stress

以红豇豆(Vigna ungniculatassp.sesquipedaliscv.chuangfeng)为材料,采用悬空气培法,研究红豇豆根缘细胞产生的数目、活性及对铝胁迫的响应。结果表明,红豇豆的边缘细胞数目先随着根的伸长而迅速增加,到根长为10mm时达到最多,约为5300个;随后,边缘细胞数目稍有减少,且较为稳定。边缘细胞的存活率较高,都大于85%,随着红豇豆根长的伸长,边缘细胞的活性逐渐增高。根冠果胶甲基酯酶(PME)的活性随着根的伸长而减小,表明边缘细胞游离与根冠PME活性有着密切的相关性。离体边缘细胞的存活率随着Al3+处理浓度和处理时间的增加而降低。不同浓度Al3+液处理对相同根长红豇豆的根冠PME酶活性没有显著的影响。

Experiments were conducted with red cowpea （ Vigna ungniculata ssp. sesquipedalis cv. chuangfeng） as experimental materials to study the numbers, activity and responsiveness of root border cells to Al stress using aeroponic culture. The results indicated that root border cell number of red cowpea increased rapidly with the elongation of root. It reached peak being around 5300 when roots were 10mm, then it decreased slightly and kept rather stable. All root border cells had a high viability, which was higher than 85 %. With the extending of root, activities of board cell were promoted stepwise; while peetin methylestcrase （PME） activity in root cap was abated indicating that PME activities were closely related with root border cell separation. Root border cell＇ s viability in intro decreased with increasing of concentration of Al^3＋ and/or their access time to Al^3＋ . No significant difference on PME activities in root cap was observed between treatments treated under different Al^3＋ concentration when cowpea roots had same length.