雌激素对骨质疏松大鼠牙槽骨改建过程中IL-1表达的影响

The effect of estrogen on the expression of IL-1 in remodeling osteoporotic alveolar bone

目的:研究雌激素对骨质疏松牙槽骨改建过程中白细胞介素-1(IL-1)的分布及表达的影响,探讨雌激素对骨质疏松牙槽骨改建影响的机制。方法:利用大鼠卵巢切除术制成骨质疏松模型,并运用免疫组化染色方法及半定量分析,观察IL-1在骨质疏松及雌激素治疗后的牙槽骨中的表达变化。结果:IL-1在骨质疏松牙槽骨中的表达明显增强,阳性反应主要分布于破骨细胞、部分成骨细胞中,而经雌激素治疗后,IL-1的阳性表达明显下降。结论:雌激素对骨质疏松大鼠牙槽骨中IL-1的表达有明显的抑制作用。

Objective：To investigate the effect of estrogen on the expression and distribution of IL-1 in the remodeling osteoporotic alveolar bone. nethods：Ovariectomy was performed on 30 female SD rats and sham operation on 15 （control group）. When osteoporosis was developed in the 30 ovary-removed rats, the left maxillary molars of all the rats were extracted. On the following day 15 of them were treated with intramuscular estrogen injection at 20 μg/kg once every 3 days（ treatment group） . Another 15 rats with ovary removed received no treatment （osteoporosis group）. 7,30 and 60 days after teeth extraction 5 rats were killed respectively in each group. IL-1 expression in alveolar bone was examined immunohistochemically,and semiquantitative analysis of cellular-staining intensity was done by microphotometry. Results：The positive IL-1 expression were mainly located in the osteoclasts,vascular endothelial cells and fibroblasts. 7 days ＂after teeth extraction the cellular-staining intensity of IL-1 in control group, osteoporosis group and treatment group was 0. 11 ＋ 0. 019,0.36 ± 0. 038 and 0. 18 ± 0. 025 respectively（ control or treatment group vs osteoporosis group,P 〈0.01 ）. 30 and 60 days after teeth extraction IL-1 expression in the three groups was not significantly different. Conclusion：Estrogen can inhibit IL-1 expression in remodeling osteoporotic alveolar bone.