摘要
目的:研究雌激素对骨质疏松牙槽骨改建过程中白细胞介素-1(IL-1)的分布及表达的影响,探讨雌激素对骨质疏松牙槽骨改建影响的机制。方法:利用大鼠卵巢切除术制成骨质疏松模型,并运用免疫组化染色方法及半定量分析,观察IL-1在骨质疏松及雌激素治疗后的牙槽骨中的表达变化。结果:IL-1在骨质疏松牙槽骨中的表达明显增强,阳性反应主要分布于破骨细胞、部分成骨细胞中,而经雌激素治疗后,IL-1的阳性表达明显下降。结论:雌激素对骨质疏松大鼠牙槽骨中IL-1的表达有明显的抑制作用。
Objective:To investigate the effect of estrogen on the expression and distribution of IL-1 in the remodeling osteoporotic alveolar bone. nethods:Ovariectomy was performed on 30 female SD rats and sham operation on 15 (control group). When osteoporosis was developed in the 30 ovary-removed rats, the left maxillary molars of all the rats were extracted. On the following day 15 of them were treated with intramuscular estrogen injection at 20 μg/kg once every 3 days( treatment group) . Another 15 rats with ovary removed received no treatment (osteoporosis group). 7,30 and 60 days after teeth extraction 5 rats were killed respectively in each group. IL-1 expression in alveolar bone was examined immunohistochemically,and semiquantitative analysis of cellular-staining intensity was done by microphotometry. Results:The positive IL-1 expression were mainly located in the osteoclasts,vascular endothelial cells and fibroblasts. 7 days "after teeth extraction the cellular-staining intensity of IL-1 in control group, osteoporosis group and treatment group was 0. 11 + 0. 019,0.36 ± 0. 038 and 0. 18 ± 0. 025 respectively( control or treatment group vs osteoporosis group,P 〈0.01 ). 30 and 60 days after teeth extraction IL-1 expression in the three groups was not significantly different. Conclusion:Estrogen can inhibit IL-1 expression in remodeling osteoporotic alveolar bone.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2006年第5期684-687,共4页
Journal of Practical Stomatology
基金
陕西省2000年自然科学基础项目2000sm52