人成釉细胞瘤中蛋白激酶C-α表达与端粒酶活性的关系

Expression of protein kinase C-α and telomerase in human ameloblastoma

目的:研究蛋白激酶C-α(PKCα)在人成釉细胞瘤(AB)中的表达及与端粒酶逆转录酶(hTERT)的关系,探讨其临床生物学意义。方法:用原位杂交和免疫组化SP法分别检测了AB中hTERT mRNA及PKCα蛋白的表达。同时选取牙源性角化囊肿(OKC)16例,正常口腔黏膜7例做对比研究。结果:hTERT mRNA在AB中阳性率为94.4%(51/54),OKC中为87.5%(14/16)及1/7正常口腔黏膜有hTERT mRNA表达,组间差异有显著性(P<0.001)。伴随AB的复发与恶变hTERT的表达逐渐增高。PKCα在AB中阳性表达为85.1%(46/54),OKC中为56.2%(9/16),4/7正常口腔黏膜有PKCα阳性,组间差异有显著性(P<0.01),伴随AB的复发与恶变PKCα阳性表达也随之增高(P<0.05)。hTERT阳性表达与PKCα阳性表达之间经Kendall相关分析rk=1.000,P=0.005呈高度正相关。结论:hTERT与AB的发生发展及临床生物学行为相关,端粒酶活性的释放激活与PKCα的高表达可能正相关。

Objective： To investigate the expression of protein kinase C-α （PKC-α） in human ameloblastoma （AB） and its relationship with human telomerase reverse transcriptase （hTERT）. Methods： The expression of hTERT mRNA was detected by in situ hybridization and PKC-α protein by immunohistochemistry in 54 cases of AB, 7 cases of normal oral mucosa（NOM） and 16 cases of odontogenic keratocyst （ OKC ）. Results ： The positive rate ofhTERTmRNAinAB, OKC and NOM was 94.4%（51/54）, 87.5%（14/16） and 14.3%（1/7）（P〈 0.001 ） respectively. The positive rate of PKC-α in AB, OKC and NOM was 85. 1% （46/54）, 56.2% （9/16） and 57.1% （4/7） （ P 〈0.01 ） respectively. In recurrent and malignant AB both hTERT and PKC-α expressions were higher than those in primary AB（P 〈0.05 ）. hTERT and PKC-α showed positive relation proved by Kendall correlation analyze （rk= 1. 000 ,P = 0. 005 ）. Conclusion：hTERT plays an important role in genesis and development of AB. Telomerase activity is positively correlatied with PKC-α expression in AB.