HSP70在大鼠牙移动过程中的表达及其生物学意义

The expression of HSP70 during experimental tooth movement in rats.

目的:研究大鼠磨牙移动过程中诱导型热休克蛋白70(HSP70)在牙周组织中的动态表达特点,推测其在牙周组织改建中的生物学意义。方法:建立大鼠牙移动的动物模型,采用免疫组织化学方法观察大鼠磨牙移动1、3、5、7、14d内诱导型HSP70在牙周组织中的动态表达及定位情况。结果:HSP70的表达在牙周膜受力早期呈强阳性,随后表达逐渐减弱,至14d为弱阳性;同一时期牙周膜的张力侧和压力侧诱导型HSP70的表达存在差异。结论:HSP70在大鼠磨牙受外力移动过程中呈现由强到弱的动态变化及区域性变化,这可能与牙周组织损伤的应激保护和改建过程中蛋白合成的需要有关。

Objective：To observe HSP70 expression during experimental tooth movement in rats. Methods： Orthodontic appliance was placed between the maxillary right first molar and maxillary central incisors of 30 adult SD rats. The rats were killed at 1,3,5,7,14 d after orthodontic force applieation, samples of experimental teeth with periodontal tissue were prepared for immunohistochemical examination of HSP70 expression. Result：Strong expression of HSP 70 in periodontal ligment was observed at 1 and 3 d after orthodontic force application, positive at 5 d and weak positive at 7 and 14 d, Conclusion： HSP 70 is expressed in periodontal ligment at different levels in different stages during orthodontic tooth movement.