多重PCR检测食品中转基因成分研究

STUDY OF MULTIPLEX-PCR FOR THE DETECTION OF GENETICALLY MODIFIED CONTENTS IN FOOD

〔目的〕建立检测转基因食品的多重PCR—聚丙烯酰胺凝胶电泳快速检测体系。〔方法〕针对转基因大豆、玉米、油菜的多个相对稳定的转基因元件,包括35S、NOS、EPSPS、Cry1A、NPTⅡ等基因,同时选定植物本身固有的大豆Lectin、玉米IVR、油菜Napin基因作为内源参照指示基因,设计、筛选出9对引物分别组成多重PCR,结合高灵敏度的聚丙烯酰胺凝胶电泳组成快速检测体系,对转基因食品进行检测,1￣2天能完成整个检测过程。对珠海地区市售的大豆、玉米、油菜及其加工产品共185个样品中的转基因成分进行了初步调查。〔结果〕建立的多重PCR检测体系稳定可靠、特异性好,灵敏度高。调查的185份可疑食品样品中,转基因阳性率达27.6%。〔结论〕该检测体系快速可靠、灵敏准确、特异性好,且操作简便、成本低廉,是一种进行转基因食品检测的良好的技术模式。对珠海地区的转基因食品状况有了一定了解。

A rapid multiplex PCR-polyactylamide gel electrophoresis （PAGE） system was developed to detect genetically modified food. The genes modified steadily （35S, NOS, EPSPS, Cry1A and NPTⅡ） in transgenic soybean, corn, cole, and the inherent control genes in plants （soybean Lectin, corn IVR and cole Napin） were selected as the target genes. And then nine primer pairs for the multiplex PCR were designed and screened out. A rapid detection system was developed for the transgenic food, which will take only 1-2 days for the entire detection, by combining the multiplex PCR with the high sensitive polyactylamide gel electrophoresis （PAGE）. 185 commercial food samples in Zhuhai were detected to investigate whether they were genetically modified, and the ratio of positive samples was 27.6%. The method was proved to be simple, rapid and sensitive with low cost. We also know the generals of the genetically modified foods in Zhuhai by this test.