摘要
目的:建立加替沙星的反相高效液相色谱方法,并对其肾损害模型大鼠体内过程进行分析研究。方法:正常和肾损害造模大鼠ig 20 mg·kg-1加替沙星后5,15 min和4 h测定各组织药物浓度,并收集尿液、胆汁与粪便,测定累积排泄率。结果:正常和肾损害造模大鼠ig20 mg·kg-1加替沙星后5,15 min和4 h,快速分布在各组织中,其中肝、肾、小肠、胃分布最多,大脑未测到药物。正常组与肾损害组大鼠ig 20 mg·kg-1加替沙星后48 h,尿液、胆汁与粪便药物累积排泄率分别为(66．2±8．8)％与(71．2±13．6)％,(8．05±3．08)％与(1．62±O.67)％和(3．63±1．65)％与(3．92±1．87)％。结论:加替沙星在肾损害模型大鼠组织分布和尿液的排泄受到影响,通过了解其体内过程,可为临床应用提供参考。
Objective： To establish RP-HPLC method for determination of the gatifloxaein, and study in vivo tissues distribution of gatifloxaein in injured kidney of rats. Method： After a single of 20 mg·kg^-1 gatifloxacin was given to rats of normal and injured kidney by i. g,drug concentrations of tissues and accumulative excretions of the urinary, bile and dejection were determined by HPLC at 5 min, 15 min and 4 h. Result： The gatifloxaein was distributed rapidly and extensively to all tissues, especially in liver, intestine, stomach and kidney, but not determined in the brain. The accumulative excretions of he urinary, bile and dejection in normal and injured rats were （66.2±8.8）% and （30.6±12.0）%, （8.05 ±3.08）% and （12.68±6.67）%, （3.63±1.65）% and （8.22 ±3.00） %, respectively. Conclusion： The gatifloxaein was influenced in tissues distribution and excretions of the urinary by injured kidney in rats. It can be offer the reference for the clinical application.
出处
《中国药师》
CAS
2006年第10期894-897,共4页
China Pharmacist
基金
广西自然科学基金资助(编号0249016)
