高效液相色谱法测定消石利胆浓缩丸中芍药苷的含量

Determaination the Content of Paeoniflorin in Xiaoshilidan Pills by HPLC

目的:建立HPLC法测定消石利胆浓缩丸中芍药苷的含量。方法:使用Lichrosorb C18色谱柱(250 mm×4．6 mm,5μm),以甲醇-0．05 mol·L-1磷酸二氢钾溶液-三乙胺(28∶72∶0．5)为流动相,流速为1．0 ml·min-1,检测波长为230 nm。结果:芍药苷在0．39～0．92μg范围内呈线性关系,r=0．999 9,平均回收率为98．4％,RSD为2．0％。结论:该方法简便,重复性好,可用于该药的质量控制。

Objective： To establish a HPLC mothod for determination the content of paeoniflorin in Xiaoshflidan pills. Method. The Liehrosorb C18 column （250 mm×4.6 mm ,5μm）was used, the mobile phase consisted of Methanol-0.05 mol·L^-1 potassium dihydrogen phosphate-triethylamine （28：72：0.5 ）, the flow rate was 1.0ml. min^-1, the detection wavelength was at 230 nm. Result： The linear range of paeoniflorin was 0.39 - 0.92μg （ r = 0. 999 9 ） , the average recovery was 98.4% , RSD = 2.0%. Conclusion： The methed is simple and repeatable, it can be used for quality control of Xiao Shi Li Dan Nong Suo pills.