摘要
本试验以辽宁绒山羊和内蒙古绒山羊为实验动物,采用PCR-RFLP技术对-酪蛋白基因(CSN3)片段的Ⅰ的酶切多态性进行分析,结果表明:辽宁绒山羊和内蒙古绒山羊2个山羊群体中均存在CSN3基因Ⅰ酶切位点的多态性,且均有A、B两个等位基因。辽宁绒山羊群体等位基因A和B的基因频率分别为0.46和0.54;内蒙古绒山羊群体中等位基因A和B的基因频率分别为0.31和0.69。CSN3基因Ⅰ酶切位点的基因型分布在辽宁绒山羊群体中极显著偏离Hardy-Weinberg平衡定理(<0.01),在内蒙古绒山羊群体中符合Hardy-Weinberg平衡定理(>0.05),但两个群体间存在显著差异(<0.01)。
By the technology of polymerase chain reaction - restriction fragment length polymorphism （ PCR - RFLP）, genetic polymorphism ofTaq I site of κ-casein gene in Liaoning cashmere goats and Neimenggu cashmere goats were tested. The result showed that the Taq I sites controlled by allele A and B were polymorphic in Liaoning cashmere goats and Neimenggu cashmere goats. The allele frequencies of A and B were 0. 46 and 0. 54 in Liaoning cashmere goats respectively; and the allele frequencies of A and B were 0.31 and 0.69 in Neimenggu cashmere goats respectively. The genotype distribution of Taq I sites in Liaoning cashmere goats didn＇t fit significantly Hardy - Weinberg Law （p〈0.01）, but the Taq I sites in Neimenggu cashmere goats fitted J-lardy - Weinberg Law （p〉0.05）, but the difference of the genotype distribution between Liaoning cashmere goats and Neimenggu cashmere goats is very significant（p 〈0.01）.
出处
《吉林畜牧兽医》
2006年第10期7-9,共3页
Jilin Animal Husbandry and Veterinary Medicine
