不同针刺时间对致敏豚鼠血清IL-4和TNF影响的实验研究

Influence of different acupuncture time on serum IL-4 and TNF levels in sensitized guinea pigs

目的对针刺大椎治疗哮喘的时效性进行初步探讨。方法以哮喘模型豚鼠为观察对象,随机分为空白组、模型组、每日针刺组、隔日针刺组和隔2日针刺组,选用大椎穴作为治疗穴位,以白细胞介素-4(IL-4)和肿瘤坏死因子(TNF)为观察指标。结果针刺大椎穴后,各针刺组IL-4含量和TNF含量均有不同程度的下降,隔2日针刺组降低最为明显,与模型组相比有非常显著的差异(P<0.001)。结论隔2日针刺为最佳治疗时间。

Objective To investigate the time-effectiveness of acupuncture in the acupoint of Dazhui （GV14） on asthma. Method The asthma model of guinea pigs were randomly divided into group 1 （the blank group）, group 2 （the model group）, group 3 （acupunctured every day）, group 4 （acupunctured every other day） and group 5 （acupunctured every three days）. The acupoint of GV14 was chosen as the treatment acupoint and the serum levels of interleukin-4 （IL-4） and tumor necrosis factor （TNF） were observed. Result After acupuncture in GV14 the serum levels of IL-4 and TNF in group 3, 4 and 5 were decreased in varying degrees. The result of group 5 was most significant and there was a great significant difference between group 5 and group 2 （P〈0.001）. Conclusion Acupuncture given every three days will have the best time-effectiveness.