利用基因枪转化法获得受四环素调控的水稻雄性不育植株

Developed Tetracycline-conditioned Male Sterile Transgenic Rice Plants by Particle Bombardment

利用基因枪法通过pTET-BI-Bar和pTATx-BN-Bin质粒分别将TetR和Barnase基因共转化粳稻品种台北309、4008S和籼稻品种D68。通过对R0代植株不同的基因进行PCR及Southern分析,结果表明,Barnase、TetR基因和控制Barnase基因的TA29-TX启动子均已整合到水稻基因组。其中,台北309和4008S分别获得了15株和6株阳性转基因植株,其外源基因转化率分别为4.5%和1.9%,其外源基因TetR和Barnase的共转化率分别为0.6%和0.3%。D68没有获得阳性转基因植株。

Taipei 309 （japonica）, 4008S （japonica） and D68 （indica） were co-transformed with Barnase and TetR genes via the plasmids of pTET - BI - Bar and pTATx - BN - Bin by particle bombardment. The PCR and southern blot analyses of each gene in R0 plants showed that the Barnase, TetR genes and TA29 - TX promoter were successfully integrated into the genome of transgenic plants. 6 and 15 transgenic plants of 4008S and Taipei 309 were obtained, respectively. The gene transformation frequency of 4008S and Taipei 309 were 1.9 % and 4.5 %, respectively, and the efficiency of co-transformation of both TetR and Barnase genes of 4008S and Taipei 309 were 0. 3 % and 0.6 %, respectively. However, no transgenic plants were found in the indica variety D68.