摘要
采用免疫组织化学和原位杂交方法，研究自由基（ＦＲ）对猪主动脉内皮细胞（ＥＣ）的ｃ－ｓｉｓｍＲＮＡ及血小板源生长因子（ＰＤＧＦ）Ｂ链蛋白表达的影响。结果显示，ＦＲ促进ｃ－ｓｉｓｍＲＮＡ和ＰＤＧＦ－Ｂ链蛋白的表达。ＥＣ暴露于ＦＲ２０ｍｉｎ，ｃ－ｓｉｓｍＲＮＡ和ＰＤＧＦ－Ｂ链蛋白的表达较对照组分别增加１．０和２．２倍。提示，ＦＲ损伤ＥＣ所诱导的ＰＤＧＦ合成增多在动脉粥样硬化的发病过程中可能起重要作用。
The effects of free radicals (FR) on the expression of c-sis mRNA and platelet-derived growth factor(PDGF)B chain protein in porcine aortic endothelial cells(EC)were studied with immunohistochemistry and in situ hybridization metheds.The results showed that FR enhanced the c-sis mRNA and PDGF-B chain protein expression signicantly.Exposing EC to FR releasing system for 20 min, the expressions of c-sis mRNA and PDGF-B chain protein were increased 1.0 and 2.2 times respectively as compared with that of the control group.These findings suggest that the synthesis of PDGF may be increased through damaging EC by FR, which in turn may play an important role in the pathogenesis of atherosclerosis.
出处
《同济医科大学学报》
CSCD
北大核心
1996年第5期338-340,共3页
Acta Universitatis Medicinae Tongji
基金
国家自然科学基金
关键词
内皮细胞
自由基
血小板源
生长因子
原位杂交
endothelial cell
free radical
platelet-derived growth factor
atherosclerosis
immunohistochemistry
in situ hybridization
