玫瑰海棠组培快繁技术的研究

Study on tissue culture and rapid propagation of Begonia mannii

采用盆栽玫瑰海棠的叶片作外植体进行组织培养试验。结果表明：用0．1％HgCl2对叶片表面灭菌10min的死亡率和感染率较低，接种于3／4MS＋6-BA2．5mg／L＋NAA1．0mg／L＋琼脂7．8g／L＋蔗糖25g／L的培养基上诱导出新生芽，在3／4MS＋6-BA1．0mg／L＋NAA0．2mg／L＋琼脂7．8g／L＋蔗糖25g／L的培养基上，增殖系数可达6～8；在1／2MS＋KT2．0mg／L＋NAA0．3mg／L＋琼脂7．8g／L＋蔗糖25g／L的培养基上培养25d左右，可形成6～9条粗壮的根和2．0～3．0cm高的完整植株，试管小苗带培养基移栽到泥炭＋泥沙＋锯屑（1：1：1）的基质上，成活率达95％。

Tissues cultures were carried out using the blade of Begonia rnannii in pot as explants. Results showed that explants of blade＇s which their surfaces treated by 0. 1 % HgCl2 for 10 min could obtain low rate of dead and infection, and they could be induced to regenerate plant when they were inoculated in culture medium of 3/4 MS＋6-BA 2.5 mg/L＋NAA 1.0 mg/L＋Agar 7.8 g/L＋Sucrose 25 g/L. The regenerated plant of B. rnannii cultured in 3/4 MS＋6-BA 1.0 mg/L＋NAA 0.2 mg/l.＋ Agar7. 8 g/L ＋ Sucrose25 g/Lhadthepropagation coefficient of 6 ～ 8. An2. 0～ 3. 0 cm individual plant with 6～ 9 strong roots was obtained after the regenerated plants were divided and cultured in 1/2 MS＋KT 2.0 mg/L＋NAA 0.3 mg/L＋Agar 7.8 g/L＋Sucrose 25 g/L for 25 days. The optium transplantation medium is Peat＋Silt＋Sawdust （1 ： 1 ： 1） in which the survival rate of transplant test-tube seedling is 95%.