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人类高度进化保守基因hnulp1中转录抑制区段的定位 被引量：3

Mapping of the Transcription Repressive Domain in the Highly Conserved Human hnulp1 Gene
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摘要 hnulp1是具有碱性螺旋-环-螺旋(bHLH)的新的一类转录因子.其C端含一个DUF654结构域,其序列在同源基因中相当保守,但该结构域功能未知.利用GAL4转录因子中的DNA结合结构域(DBD)和含有与DBD结合序列的荧光素酶报告基因(GAL4-Luc)质粒,构建了哺乳动物细胞转录因子活性分析系统,随后利用GAL4-Luc荧光素酶报告基因对5种含DUF654结构域的不同缺失片段转录抑制活性进行检测.检测结果表明,该基因DUF654结构域中从Δ228-407氨基酸区段具有强烈的转录抑制活性.该结果为进一步研究DUF654结构域的功能和hnulp1基因转录调控的机制奠定了基础. hnulp1 is a new member of basic helix-loop-helix transcription factors. It contains a DUF654 domain in C-terminus, which is highly conserved from yeast, zebrafish, chicken, A. thaliana, C. elegans, Drosophilae to mouse. However, the function of this motif is currently unknown. Five deletion fragments of DUF654 domain were fused to GAL4 DNA-binding domain, and then co-transfected with L8G5-Luc and VP-16 plasmids. The analysis of the GAL4 luciferase reporter gene indicated that fragments from 228 to 407 amino acids of DUF654 domain have strong transcription suppression activity. Solid basis is layed for the hnulp1 transcriptional regulation mechanism and the function of DUF654 domain.
作者 刘连 蔡振宇 王跃群 吴秀山
机构地区 湖南师范大学生命科学学院蛋白质化学及发育生物学教育部重点实验室
出处 《生命科学研究》 CAS CSCD 2006年第3期194-199,共6页 Life Science Research
基金 国家自然科学基金项目(3021010392 30270722 30270644 30470867 30570265) 科技部973项目(2005CB522505) 长江学者创新团队发展计划(IRT0445) 湖南省科技厅基金(05FJ2007)
关键词 DUF654 荧光素酶报告基因 转录活性 hnulp1 DUF654 luciferase reporter gene transcription activity hnulp1
分类号 Q132.3 [生物学—普通生物学]
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参考文献6

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同被引文献15

  • 1蔡振宇,袁婺洲,吴秀山.心肌-内皮信号途径在心脏发育中的作用[J].生命科学研究,2003,7(S1):6-11. 被引量：4
  • 2吴樱,黄鹤,周加文,甘一如.利用pET-29b载体进行葡激酶基因表达与其产物的亲和纯化[J].吉林大学学报（医学版）,2004,30(6):865-867. 被引量：3
  • 3刘海英,杨微,武正华,唐晓波,朱大岭.细胞色素P450 3A4抗原表位的分析及其抗体的制备与鉴定[J].细胞与分子免疫学杂志,2007,23(1):79-81. 被引量：8
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  • 5MAGNUS O, MADELEINE D, PETER E, et al. Nulpl, a novel basic helix-loop-helix protein expressed broadly during early embryonic organogenesis and prominently in developing dorsal root ganglia[ J ]. Cell Tissue Res, 2002, 308:361-370.
  • 6HAKAN S, DAN L. Nuclear localized protein-1 ( Nulpl ) increases cell death of human osteosarcoma cells and binds the X- linked inhibitor of apoptosis protein[ J]. BBRC, 2008, 336:432-437.
  • 7CAI Z, WANG Y, XIAO J, et al. Hnulpl, a basic helix-loop-helix protein with a novel transcriptional repressive domain, inhibits transcriptional activity of serum response factor[ J]. BBRC, 2006, 343(3) :973-981.
  • 8MARK E M, CORNELISM. Helix- loop- helix proteins: regulators of transcription in eukaryotic organisms [ J ]. Mol Cell Biol, 2000, 20 (2): 429-440.
  • 9MAGNUS O, MADELEINE D, PETER E, et al. Nulpl, a novel basic helix- loop- helix protein expressed broadly during early embryonic organogenesis and prominently in developing dorsal root ganglia [J]. Cell Tissue Res, 2002, 308:361 - 370.
  • 10HAKAN S, DAN L. Nuclear localized protein - 1 ( Nulpl ) increases cell death of human osteosarcoma ceils and binds the X linked inhibitor of apoptosis protein [J]. BBRC, 2008, 336 432 - 437.

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生命科学研究
2006年 第3期

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