青龙藤提取物对糖尿病模型大鼠的降血糖作用

Effect of Biondia Heriyi extract on blood glucose level in Streptozocin-induced diabetic rats

目的:探讨青龙藤提取物(BH)降低糖尿病模型大鼠的血糖作用。方法:将32只雄性Wistar大鼠随机均分成4组,正常对照组,其他3组以链脲佐菌素(STZ)制成接近于人1型糖尿病的大鼠糖尿病模型。3d后经血糖测试仪检测,血糖含量>16·65mmol·L-1为制模成功。1组作为糖尿病模型对照组,另外2组7d后每天分别灌胃给予BH(465mg·kg-1)和消渴丸(XKW,625mg·kg-1),连续5周给药。每周1次测定给药过程中各组大鼠血糖变化。注射STZ3d和实验结束后,采用放射免疫分析方法分别测定各组大鼠血清中胰岛素含量。实验结束后观察各组大鼠胰岛β细胞的形态变化。结果:给予BH和消渴丸5周后,糖尿病BH给药组大鼠血糖由(18·3±1·8)mmol·L-1下降到(5·3±1·2)mmol·L-1,消渴丸对照组血糖由(18·4±1·9)mmol·L-1下降到(5·2±0·8)mmol·L-1,两组与糖尿病模型对照组[(19·9±1·9)mmol·L-1]比较差异均有显著性(P<0·001)。糖尿病BH给药组大鼠血清胰岛素含量由(18·8±2·3)mU·L-1升高到(42·2±3·5)mU·L-1,消渴丸组大鼠血清胰岛素含量由(18·1±3·3)mU·L-1升高到(40·9±3·7)mU·L-1,两组与糖尿病模型对照组[(18·7±3·1)mU·L-1]比较差异均有显著性(P<0·01),且基本接近正常对照组。糖尿病模型组大鼠的胰岛变小,细胞萎缩,胞核固缩、消失,分泌颗粒减少。应用BH和消渴丸5周后,上述异常变化消失且胰岛细胞形态趋向正常。结论:BH对糖尿病大鼠有降低血糖、升高血清胰岛素、恢复和保护胰岛β细胞的作用。

Abstract： Objective To investigate the effect of Biondia Heriyi （BH） extract on Streptozocin （STZ） -induced diabetic rats. Methods Thirty two male rats were divided randomly into four groups： control group （A） and three groups of diabetic models. The diabetic model was established after three days according to blood glucose level （〉16.65 mmol ·L^-1 ） measured by blood glucose analyzer. Then the diabetic rats were divided into three group, one was control group of diabetic rats, the other two groups were received BH （465 mg · kg^-1） and XKW （625 mg · kg^-1） into stomach respectively, the glucose concentration in blood was measured once a week. For all the three groups of diabetic rats, at the three days after injection of STZ and the end of the experiment, the insulin concentrations in blood were determined by radioimmunoassay. The morphological structure of the pancreatic island was also observed. Results After administration of BH extract and XKW for five weeks respectively , the level of blood glucose in diabetic rats of BH-administration group decreased from （18.3±1.8） mmol ·L^-1 to （5.3±1.2）mmol·L^-1, the level of blood glucose in diabetic rats of XKW-administration group decreased from （18.4±1.9） mmol·L^-1 to （5.2±0.8） mmol ·L^-1, compared with the level in control group of diabetic rats [ （19.9 ± 1.9） mmol ·L^-1, there was significant differente （P〈0.001 ）. The blood insulin level in diabetic rats of BH administered group was increased from （18.8±2.3） mU ·L^-1 to （42.2±3.5） mU ·L^-1. The blood insulin level in diabetic rats of XKW administration group increased from （18. 1±3. 3） mU ·L^-1 to （40. 9±3. 7） mU ·L^-1 , compared with the level in the control group of diabetic rats [ （18.7±3.1） mU ·L^-1, there was significant difference （P〈0.01）. To corresponding the changes of the blood glucose and insulin, the morphological changes of the pancreatic island cells in diabetic rats were the changes in the cell atrophy, nucleus pyknosis, secretion particles and so on which were recovered to the normal situation five weeks after BH extract and XKW were administered. Conclusion BH could effectively decrease the blood glucose and increase the insulin level of STZ-induced diabetic rats, and has effects to protect and recover the pancreatic island cells.