β-联蛋白在恶性胸膜间皮瘤组织的表达及其对肿瘤的抑制作用

Expression of β-catenin on diffused malignant plural mesothelioma tissues and its inhibitory effects

目的:探讨β-联蛋白在恶性胸膜间皮瘤组织的表达及其对肿瘤抑制作用。方法:应用AnnexinV和TUNEL法分别检测转染野生型β-联蛋白(β-CAT-V5-FP)和突变型β-联蛋白(β-CAT-S37C-V5-FP)诱导恶性胸膜间皮瘤NCI-H28细胞凋亡;采用体外细胞培养法,研究β-联蛋白基因对肿瘤的抑制作用。结果:转染野生型β-联蛋白和突变型β-联蛋白的细胞凋亡率与转染空白载体组比较均有明显的增高。转染羧基端缺失的β-联蛋白基因也可导致细胞凋亡,但与转染野生型β-联蛋白(β-CAT-V5-FP)和突变型β-联蛋白(β-CAT-S37C-V5-FP)的细胞相比明显减少。结论:β-联蛋白基因在非上皮性肿瘤——恶性胸膜间皮瘤中也可诱导细胞凋亡。

Objective To study the β-catenin gene expression and inhibitory effects on diffused malignant plural mesothelioma tissues. Methods NCI-H28 cell apoptosis was detected with the techniques of Annexin V and TUNEI.. The inhibitory effect of β-catenin was observed with incubation in vitro. Results The cells transferring wild β-catenin （β-CAT-V5-FP） and mutant β-catenin （β-CAT-S37C-V5-FP） were increased signifcantly compared with blank carrier group. The β-catenin gene transferring carboxyl absent also resulted in apoptosis, but the apoptotic rate was lower than those of cells transferring wild β-catenin （β-CAT-V5-FP） and mutant β-catenin （β-CAT-S37C-V5-FP）. Conclusion β-catenin gene can induce apoptosis on non-epithelioid tumor-diffused malignant plural mesothelioma.