妊娠期高血压疾病患者血管紧张素Ⅱ1型受体基因多态性

Gene polymorphism of angiotensin Ⅱ type 1 receptor in patients with hypertensive disorder complicating pregnancy

目的:探讨血管紧张素Ⅱ1型受体(AT1R)基因非编码区(-535)位点多态性与妊娠期高血压疾病(HDCP)发病的关系。方法:采用聚合酶链反应-限制性酶切片段长度多态性(PCR-RFLP)技术检测86例HDCP组孕妇(妊娠期高血压17例、子痫前期轻度28例、子痫前期重度23例、子痫18例)和74例对照组(正常孕妇)基因型。结果:①HDCP组孕妇AT1R基因(-535)位点基因型CC、CT、TT频率分别为74·4%、20·9%和4·7%,对照组分别为87·8%、8·1%和4·1%,两组基因型频数分布比较差异无显著性(P>0·05);HDCP组孕妇AT1R基因(-535)位点等位基因C、T频率分别为84·9%和15·1%,对照组分别为91·9%和8·1%,两组等位基因频率比较差异亦无显著性(P>0·05);②HDCP组妊娠期高血压、子痫前期轻度、子痫前期重度和子痫孕妇(-535)位点T等位基因频率分别为5·9%、7·1%、15·2%和36·1%,前3者与子痫患者比较差异均有显著性(P<0·05)。结论:AT1R基因(—535)C→T变异与妊娠期高血压疾病发病无关,但与病情严重程度有关。

Objective To investigate the association between angiotensin Ⅱ type 1 receptor （AT1R） gene polymorphism and hypertensive disorder complicating pregnancy （HDCP）. Methods Eighty-six pregnant women with HDCP and 74 normal pregnant women （control group） were recruited. Gene polymorphism of AT1R at （-535） was detected with PCR based restriction fragment length polymorphism （ RFLP ） method. Results ① The frequencies of CC, CT, TT genotypes of AT1R at （-535） were 74.4%, 20.9%, 4.7% in HDCP group and 87.8%, 8.1%, 4.1% in control group, respectively; there was no significant difference berween two group （P〉0.05）. The frequencies of the two alleles C, T were 84.9%, 15. 1% in HDCP group and 91.9 %, 8.1 %in control group, respectively; there was no significant difference between two group （P〉0. 05）; ②The distribution frequencies of AT1R at （- 535） in HDCP group in gestational hypertension, mild preeclampsia, severe preeclampsia （5.9%, 7.1%, 15.2% were lower than that in eclampsia in HDCP group （P〈0. 05）.Conclusion The AT3 R （-535） C→T gene polymorphism is not associated with the pathogenesis of HDCP, but it has an association with the severity of HDCP.