迷迭香带芽茎段的组织培养技术

Tissue Culture Techniques of Buds in Rosmarinus officinalis L.

以经种植筛选的迷迭香优株嫩芽和枝条为外植体，研究了细胞分裂素、生长素、多效唑（PP333）、三碘苯甲酸（TIBA）的不同浓度及其配比，培养基成分的浓度，附加物质椰乳（CM）及温度等对芽的诱导分化、芽的增殖、伸长及生根的影响，并研究了温度、基质对试管苗移栽成活率的影响。研究结果表明：培养基为3／4MS＋蔗糖3%＋6-BA0．2mg／L＋NAA0．01mg／L＋CM150mL／L，温度19～23℃有利于促进腋芽的形成和伸长；1／2MS＋蔗糖1．5%＋IBA1．0mg／L＋NAA0．5mg／L＋PP3330．05mg／L有利于诱导芽生根；在试管苗移栽阶段，基质选用粗河沙或粗河沙＋珍珠岩（1：1），于春天和冬天移栽，可提高试管苗移栽的成活率，使移栽成活率达到90％左右。

Taken stem sections with shoots in Rosmarinus officinalis L. as materials, effects of different media supplemented with different concentrations and proportions of KT, 6-BA, NAA, IBA, PP333 and TIBA, different concentrations of fundamental components in media, adding CM, and temperature, and so on, on inducing bud differentiating, multiplying, growing and rooting were researched, otherwise, effects of temperature and media on transplanting survival rate of test tube seedlings were researched. The results showed that the best medium for bud inducing and multiplying was 3/4MS supplemented with sucrose 3%, 6-BA 0. 2 mg/L, NAA 0. 01 mg/L and CM 150 mL/L, and culture temperature was at 23～19℃; the best medium for rooting was 1/2MS supplemented with sucrose 1.5%, IBA 1.0 mg/L, NAA0.5 mg/L, PPaaa 0. 05 mg/L; it could improve survival rate of seedlings to chose coarse sand or coarse sand and perlite （1 ： 1） as media and transplant test tube seedlings in spring and autumn, and the rate was about 90%.