摘要
目的:从淮山药中提取粗多糖RDP,进行纯化分离,得到均一的多糖RP,并初步研究其组成和结构。方法:依次经水浸提、乙醇沉淀、十六烷基三甲基溴胺盐脱蛋白、微晶纤维素柱色谱、Sephadex G-100色谱分离纯化得白色粉末状多糖RP。Sephadex G-200柱层析进行纯度鉴定。纸层析分析多糖酸水解产物;凝胶色谱法测定其相对分子质量;用红外光谱分析鉴定RP。结果:纯度鉴定证明其为均一组分;红外光谱分析其具有β-糖苷键;PC分析其单糖组成为葡萄糖、D-甘露糖、D-半乳糖。结论:本实验对为该多糖的进一步开发利用提供一定的理论依据。
Objective: To isolate a homogeneous polysaccharide RP from RDP (a crude polysaccharide from Dioscorea opposita Thunb, ), and study its preliminary composition and structure. Methods: RP was obtained with water extraction, alcohol sedimentation, CTAB deprotein, cellulose column and SephadexG-100 column. The purities of RP were identified by SephadexG-200. PC analysis on its acidic hydrolysates was used to determine the sugar components. Sephadex chromatography was used to mensurate its molecular weight. IR was used to analysis RP. Results: RP was homogeneous. IR indicated that RP had β configurations. The eompositon was identified by paper chromatography as glucose, D-mannose, D-galactose. Conclusion: The research could provide a theoretical foundation for further development and utilization.
出处
《中药材》
CAS
CSCD
北大核心
2006年第9期909-912,共4页
Journal of Chinese Medicinal Materials
基金
江苏省基础研究计划(自然科学基金)BK2003065资助项目
关键词
淮山药
多糖
Dioscorea opposita Thunb
Polysaeeharide