肾康丸对高糖培养的大鼠系膜细胞TGF-β_1及FN的影响

Effect of Shenkangwan on TGF-β_1 and FN in Rat Mesangial Cells Cultured by High Glucose

目的:观察肾康丸对高糖培养大鼠系膜细胞(MC)分泌转化生长因子(TGF-β1,及纤连蛋白(FN)分泌mRNA表达的影响,探讨其防治DN的分子机制。方法:体外培养大鼠MC,将细胞分为6组:低糖组、高糖组、正常血清组、开搏通组、肾康丸高、低剂量组。培养72 h后,采用双抗夹心酶联免疫吸附(ELISA)法检测高糖对MC分泌TGF-β1和FN的影响;采用逆转录-多聚酶链反应(RT-PCR)法检测高糖对TGF-β1和FN mRNA的表达的影响;同时研究药物血清对上述指标的影响。结果:高糖可以促进MC分泌TGF-β1和FN,并提高MC中TGF-β1和FN mR-NA的表达,肾康丸可以明显抑制上述作用。结论:肾康丸可以通过TGF-β1途径抑制MC基质分泌。

Objective： To investigate the effect of Shenkangwan on secretion of transforming growth factor-β1 （TGF-β1 ） and Fibronectin（ FN ）and their mRNA expression in rat mesangial cells（ MC ）cultured by high glucose and explore its molecule mechanism on treating diabetic nephropathy（DN）. Methods： Rat MC were cultured in vivo and divided into six groups： low glucose group, hight glucose group, normal rat serum group, Capoten tablets group, Shenkangwan high dose group and low dose group. Seventy two hours later, the secretion of TGF-β1 and FN in MC were detected by enzyme linked immunosorbent assay（ELISA）. The expression of TGF-β1 and FN mRNA were determined by semi-quantitative reverse transcription-polymerase chain reaction（RT-PCR）. Results： High gluscose could induce secretion of TGF-β1 and FN in MC and increase expression of TGF-β1 and FN mRNA. While Shenkangwan could repress these effects. Conclusion： Shenkangwan can suppress the secretion of the extracellular matrix in MC via TGF-β1 signal way.