药理实验中Caco-2细胞模型建立的评价指标

The Evaluation Criterions about Caco-2 Cell Model

目的:建立Caco-2细胞模型并对该模型进行评价。方法:通过显微镜观察细胞形态学特点,测定跨上皮细胞膜电阻,碱性磷酸酶活性和标志物普萘洛尔的转运特性等指标对Caco-2细胞模型进行评价。结果:各项指标的测定值与文献相符。本实验建立的Caco-2细胞模型的完整性、紧密性、通透性以及碱性磷酸酶活性较好。结论:建立的Caco-2细胞模型符合各项指标的要求,可用于研究口服药物转运的吸收机制。

Objective： To establish and evaluate the Caco-2 cell model. Methods： Caco-2 cell model was evaluated by morphology feature, TEER alkaline phosphatase and the transport of Propranolol. Results ： The determined values were corresponding to the references. The integrality, permeability and the expression of alkaline phosphatase in the cell model were superior. Conclusion ： The established Caco-2 cell model can be used to study the absorption mechanism of oral administration drug.