正常与BPH前列腺生长因子的研究

STUDIES ON PROSTATIC GROWTH FACTOR IN NORMAL AND BENIGN HYPERPLASTIC HUMAN PROSTATES

收集正常的及良性前列腺增生症（ＢＰＨ）的前列腺组织，用细胞培养、同位素掺入法分别测定各例标本的组织液对Ｓｗｉｓｓ３Ｔ３细胞增殖的影响。结果表明，ＢＰＨ组织液促细胞增殖效应为正常前列腺的５倍。正常前列腺组织用硫酸铵分级沉淀、肝素亲和层析、ＮａＣｌ浓度梯度洗脱（收集１．３～１．７ｍｏｌ部分），分离纯化人前列腺生长因子（ｈＰＧＦ）。经ＳＤＳ－ＰＡＧＥ鉴定达电泳纯，分子量约为１７０００，纯化约８００倍。所得的ｈＰＧＦ对Ｓｗｉｓｓ３Ｔ３细胞的增殖刺激作用呈现浓度依赖性。

Recent studies have focused on the potential role of human prostatic growth factor(hPGF) in the pathogenesis of benign prostatic hyperplasia(BPH). Growth factors.as detected by DNA synthesis stimulating activity for Swiss 3T3 cells.in human normal prostates and BPH tissues were analyzed. Tissue extracts of both normal prostates and BPH significantly stimulated3 H-thymidine incorporation by Swiss 3T3 cells. The total content of growth factor(units per gram of tissue)in BPH was 5 times higher than those in normal prostates. hPGF was purified about 800-folds from alkaline homogenates of human normal prostates by a combination of ammonium sulfate precipitation and heparin affinity chromatography. The growth factor was eluted from the heparin-Sepharose column at 1.3～1. 7mol NaCl. hPGF in normal prostates and BPH tissues all stimulated DNA synthesis of Swiss 3T3 cells. The stimulation was concentration dependent. Furthermore ,BPH tissues contained a significantly greater amount of hPGF activity(units/mg protein) than normal prostates(P<0. 001 ). SDS-PAGE confirmed that the partially purified hPGF had a molecular weight of about 17KDa. These findings suggest that the increased hPGF biological activity might be responsible for the development of BPH.