摘要
基于细胞的检验方法已经得到了发展，主要用于测定培养细胞中与蛋白酶体相关的糜蛋白酶样蛋白酶活性。该方法结合了发光蛋白酶检验的敏感性和单添加剂的单一性，避免了乏味的抽样过程。使用发光细胞检验法可以克服使用细胞提取物或纯蛋白酶体的局限性。能够成功获得Luminogenic蛋白酶底物的敏感性有可能直接促进“添加、混合、测定”检验法的发展，它有足够的敏感性用以直接测定多孔板细胞中的糜蛋白酶样蛋白酶活性。
Acell-based assay has been developed to measure the chymotrypsin-like protease aetivity assoeiated with the proteasome in eultured cells. Jt eombines the sensitivity of luminescent protease assays with the simplicity of a single reagent addition, eliminating tedious sample processing. Using a luminescent cell-based assay overcomes the limitations of using either cell extracts or purified proteasome preparations.The sensitivity achieved with luminogenic protease substrates has enabled the development of a direct ＂add, mix, measure＂ assay sensitive enough to measure the chymotrypsinlike proteasome activity directly in cells in multi-well plates.
