新石器时代人骨遗骸中古代DNA的提取及X-Y染色体同源基因片段的PCR扩增

ANCIENT DNA EXTRACTION FROM NEOLITHIC HUMAN SKELETAL REMAINS AND PCR BASED AMPLIFICATION OF THE X-Y HOMOLOGOUS AMELOGENIN GENE

从中国新石器时代人骨遗骸中提取出古代DNA。通过聚合酶链式反应(PCR)技术扩增得到X-Y染色体上的单拷贝同源基因片段(Amelogenin Gene)。由于扩增的基因片段长度具有性别多态性,从而为古代人骨和牙齿提供了分子生物学的性别鉴定。

Ancient DNA was extracted from Neolithic human skeletal remains excavated in China. Single copy sequences of the X- Y homologous Amelogenin gene were amplified from the DNA extracts by PCR technique. Since the amplified sequences exhibit a sex specific length dimorphism they enable a sex identification on a molecular basis for ancient human bone and teeth.