摘要
采用血凝测试及血凝活性糖抑制测试方法,测定了一种可降解黄原胶的鞘氨醇单胞菌XT-11 Sphingomonas sp.对红细胞的凝集活性及糖抑制专一性,同时进行了温度、pH及Ca^2+对红细胞凝集活性影响的试验。结果表明:XT-11菌悬液能凝集家兔、家鸡两种红细胞,其凝集效价分别为2^6和2^4;菌悬液对兔红细胞的凝集作用可以被肝素所抑制;鞘氨醇单胞菌的凝集活性不依赖于Ca^2+,且在pH为6.0~10.0时较稳定,在温度为60℃时完全丧失凝集活性。这说明该菌含有能凝集红细胞的活性物质。
Hemagglutination assay and hemagglufination inhibition assay showed that a Xanthan - Degrading Sphingomonas XT- 11 with hemagglutinating activity and hemagglutination was inhibited by sugar. Besides, thermostability and influence of pH and Ca^2+ to the activity were tested. The results showed that XT - 11 agglutinated two erythroc rin. Its hemagglutinati ytes. Agglutinative activity of XT - 11 to rabbit erythrocytes was only inhibited by hepaon activity was independent of Ca^2+ concentration. The activity of XT - 11 solution was stable in pH range from 6.0 to 10.0. The XT - 11 solution incubation at 60℃ for 30 min leaded to complete irreversible inactivation, indicating that XT - 11 contains agglutinin
出处
《大连水产学院学报》
CSCD
北大核心
2006年第3期251-254,共4页
Journal of Dalian Fisheries University
基金
辽宁省自然科学基金资助项目(2050885)
辽宁省教育厅资助项目(05L086)
关键词
黄原胶
鞘氨醇单胞菌
红细胞
凝集活性
Xanthan - Degrading
Sphingomonas sp. erythrocyte
hemagglutinating activity