一种可降解黄原胶的鞘氨醇单胞菌的凝集活性

Hemagglutination activity of a Xanthan-Degrading Sphingomonas sp.XT-11

采用血凝测试及血凝活性糖抑制测试方法，测定了一种可降解黄原胶的鞘氨醇单胞菌XT-11 Sphingomonas sp．对红细胞的凝集活性及糖抑制专一性，同时进行了温度、pH及Ca^2＋对红细胞凝集活性影响的试验。结果表明：XT-11菌悬液能凝集家兔、家鸡两种红细胞，其凝集效价分别为2^6和2^4；菌悬液对兔红细胞的凝集作用可以被肝素所抑制；鞘氨醇单胞菌的凝集活性不依赖于Ca^2＋，且在pH为6．0～10．0时较稳定，在温度为60℃时完全丧失凝集活性。这说明该菌含有能凝集红细胞的活性物质。

Hemagglutination assay and hemagglufination inhibition assay showed that a Xanthan - Degrading Sphingomonas XT- 11 with hemagglutinating activity and hemagglutination was inhibited by sugar. Besides, thermostability and influence of pH and Ca^2＋ to the activity were tested. The results showed that XT - 11 agglutinated two erythroc rin. Its hemagglutinati ytes. Agglutinative activity of XT - 11 to rabbit erythrocytes was only inhibited by hepaon activity was independent of Ca^2＋ concentration. The activity of XT - 11 solution was stable in pH range from 6.0 to 10.0. The XT - 11 solution incubation at 60℃ for 30 min leaded to complete irreversible inactivation, indicating that XT - 11 contains agglutinin