胆囊胆固醇结石患者肝脏受体类似物1基因表达差异的研究

Expression of liver receptor homolog 1 gene in patients with cholesterol gallstone disease

目的研究胆囊胆固醇结石患者肝脏受体类似物1(LRH-1)表达,探讨其与胆固醇结石病发病的关系。方法27例胆囊胆固醇结石患者,男6例,女21例,平均年龄52．44岁。14例无胆石症者为对照(肝脏肿瘤2例,胆囊息肉患者12例),男9例,女5例,平均年龄47．50岁。测定胆汁脂类成分和计算胆汁胆固醇饱和指数。实时定量PCR法测定肝脏LRH-1 mRNA的表达量。结果胆石组LRH-1表达14．18±9．37,高于对照组7．86±6．19,(P＜0．05),胆石组胆汁呈胆固醇过饱和(1．17±0．27)。结论本研究提示肝脏LRH-1表达增高与胆囊胆固醇结石病有关。

Objective To investigate the mRNA expression of liver receptor homolog 1 （LRH-1） gene in patients with cholesterol gallstone disease so that to elucidate the biomolecular pathogenesis of gallstone formation. Methods Twenty-seven patients with cholesterol gallstone （CGS） and 14 controls were included in this study. Biliary composition was assayed and mRNA expression of hepatic LRH 1 gene was determined by real-time polymorphism chain reaction. Results In CGS patients, expression of LRH-1 was significantly higher than that in controls （14.18 ± 9.37 vs 7.86 ± 6.19, P 〈 0.05）, and cholesterol of bile was oversaturated （1.17±0. 27）. Conclusion The formation of CGS may be related to increased expression of hepatic LRH-1 gene.