MNP-hTR复合物对K562细胞增殖影响的实验研究

The study on the effect of the MNP-hTR compound on the Apoptosis and proliferation of K562 Cells

探讨MNP-端粒酶反义寡核苷酸复合物,对K562细胞凋亡和增殖的影响。应用荧光显微镜检测法和集落形成法,分别观察了凋亡细胞的形态学变化和细胞增殖能力。MNP-端粒酶反义核酸复合物可诱导K562细胞凋亡和抑制细胞增殖,两实验组数值经统计学处理,具有非常显著性差异(p分别<0.001)。由此认为,MNP-端粒酶反义核酸复合物对K562白血病细胞具有促进凋亡的作用,此效果与剂量呈依赖性关系(r=0.992)。

To investigate the influence of the magnetic nanoparticles （MNP） - tolemorase antiense ohgonucleotide compound to the K562 cells, especially about the apoptosis and proliferation. Fluorescence microscope was used to watch the changing morphological characters of K562 cells, and we also used the clonoly forming unit to detect the proliferation. The apoptosis and inhibit the proliferation of K562 cells might was induced by the MNP - tolemorase antisense oligonucleotide compound. According to the statistical analysis of the experimental data, the difference is significantly （p 〈 0.01 - 0.001） compared with the control groups, respectively. The MNP - tolemorase antisense oligonucleotide compound can promote cell apoptosis, besides, the effect depends on the dosage of the comound strictly（r= 0.998）.