期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

RNA干扰技术抑制病毒复制的研究进展

Advances in the Inhibition of Viral Replication by RNA Interference
下载PDF
导出
摘要 RNA干扰是指dsRNA抑制细胞内同源基因表达的现象,RNA干扰现象自发现以来在短短的几年里,已成功地用于不同种属的生物研究。dsRNA不仅参与内源基因表达调控,而且能够抑制宿主内病原微生物基因的表达,参与构筑生物体的防御机制。近年来,运用RNAi技术在哺乳动物中的研究不断深入,尤其是抑制病毒复制的研究成果令人欣喜,这为人类动物抗病毒治疗提供了新的思路。 RNA interference refers to the inhibition of gene expression by dsRNA. The RNA interference technique has already used to the dissimilarity kinds of creature researches successfully in very short years. DsRNA can not only participate in gene regulation, but also inhibit gene expression of pathogenic microbes. RNAi is beheved to function as a defense against viruses. Recently, inhibition of virus replication by means of induced RNAi has been reported for many human and animal pathogens. It makes possible the development of RNAi - based antiviral therapeutics. In this article, we review the current researches on RNAi - mediated inhibition of virus replication.
作者 姜法铭 缪德年 崔立
机构地区 上海交通大学农业与生物学院 上海市农科院畜牧所
出处 《现代生物医学进展》 CAS 2006年第5期44-46,共3页 Progress in Modern Biomedicine
关键词 RNA干扰 DSRNA 抑制病毒复制 抗病毒治疗 RNAi dsRNA inhibition of virus replication anfiviral therapeutics
分类号 Q81 [生物学—生物工程]
  • 相关文献

参考文献29

  • 1McRobert L,McConkey GA.RNA interference (RNAi) inhibits growth of Plasmodium falciparum[J].Mol.Biochem.Parasitol,2002,119:273-278
  • 2Al-Anouti F,Quach T,Ananvoranich S.Double-stranded RNA can mediate the suppression of uracil phosphoribosyltransferase expression in Toxoplasma goudii[J].Biochem.Biophys.Res.Commun,2003,302:316-323
  • 3Ullu E,Tschudi C,Chakraborty T.RNA interference in protozoan parasites[J].Cell Microbiol,2004,6(6):509-519
  • 4Robinson KA,Beverley SM.Improvements in transfection efficiency and tests of RNA interference (RNAi) approaches in the protozoan parasite Leis mania[J].Mol Biochem Parasitol,2003,128:217-228
  • 5Stauber M,Taubert H,Schmidt-Ott U.Function of biocide and hunchback homolog in the basal cyclorrhaphan fly Megaselia (Phoridae)[J].Proc.Natl.Acad.Sci.USA.2000,97:10844-10849
  • 6Hammond SM,Berstein E,Beach D,et al.An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells[J].Nature,2000,404:293-296
  • 7Kennerdell JR,Carthew RW.Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway[J].Cell,1998,95:1017-1026
  • 8Misquitta L,Paterson BM.Targeted disruption of gene function in Drosophila by RNA interference (RNA-i):a role for nautilus in embryonic somatic muscle formation[J].Proc.Natl.Acad.Sci.USA,1999,96:1451-1456
  • 9Brooks DR,Isaac RE.Functional genomics of parasitic worms:the dawn of a new era[J].Parasitol.Int,2002,51:319-325
  • 10Caplen NJ,Parrish S,Imam F,et al.Specific inhibition of gene expression by small double-stranded RNA in invertebrate and vertebrate systems[J].Proc.Natl.Acad.Sci.USA,2001,98:9742-9747

二级参考文献69

  • 1Mast, E.E.,MJ. Aoter & H.S. Margolis, 1999, Strategies to prevent and control hepatitis B and C vires infections:a global perspective. Vaccine, 17(13-14): 1730-1733.
  • 2Liaw, Y.F. ,2002, Therapy of chronic hepatitis B: current challenges and opportunities. J. Viral Hepat, 9(6): 393-399.
  • 3Goodarzi, G., S.C. Gross, A. Tewari & K. Watabe, 1990,Antisense oligodeoxyribonucleotides inhibit the expression of the gene for hepatitis B virus surface antigen.J. Gen. Virol., 71 ( Pt 12): 3021-3025.
  • 4Wu, G.Y. & C.H. Wu, 1992, Specific inhibition of hepatitis B viral gene expression in vitro by targeted antisense oligonucleotides. J. Biol. Chem., 267(18): 12436-12439.
  • 5Wu, J. & M.A. Gerber,1997, The inhibitory of antisense RNA on hepatitis B virus surface antigen synthesis. J.Gen. Virol., 78 ( Pt 3): 641-647.
  • 6zu Putlitz J, S. Wieland, HE. Blum & JR. Wands,1998, Antisense RNA complementary to hepatitis B virus specifically inhibits viral replication. Gastroenterology, 115(3): 702-713.
  • 7Giladi, H, M. Ketzinel-Gilad, L. Rivkin, Y. Felig, O.Nussbaum & E. Galun, 2003, Small interfering RNA inhibits hepatitis B virus replication in mice. Mol.Ther., 8(5): 769-776.
  • 8Shlomai, A. & Y. Shaul, 2003, Inhibition of hepatitis B virus expression and replication by RNA interference.Hepatology., 37(4): 764-770.
  • 9Elbashir, S.M., W. Lendeckel & T. Tuschl, 2001, RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes, 15(2): 155-200.
  • 10Jiang, M. & J. Milner, 2002, Selective silencing of viral gene expression in HPV-positive human cervical carcinoma cells treated with siRNA, a primer of RNA interference. Oncogene, 21 (39): 6041-6048.

共引文献5

现代生物医学进展
2006年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部