苹果属小金海棠转录因子MxMYB1基因的克隆及其原核表达

Identification and Prokaryotic Expression of Transcription Factor MxMYB1 Gene in Malus xiaojinensis

MxMYB1是苹果属小金海棠MYB类转录因子。MxMYB1含1个重复序列及MYB蛋白特有的氨基酸组成。酶切、PCR扩增及测序分析表明构建的原核表达载体结构正确,未出现碱基突变及移码现象。1mmol/LIPTG诱导2h后,在预期的蛋白分子量38kD处出现1条表达加强的蛋白条带,而未经诱导的转化子没有此蛋白条带。为进一步目的蛋白的纯化和鉴定提供试验基础。

MxMYB1 is a MYB transcription factor from Malus xiaojinensis. MxMYB1 contains a highly conserved DNA binding domain and amino acids existed in MYB proteins particularly. Restriction endonuclease analysis, PCR amplifying and sequencing confirmed that construction was correct and had no base mutant and drift. SDS-PAGE of pET-MxMYB1 proteins induced by 1 mmol/L IPTG showed that there was a strengthened protein band in expectant 38 kD protein Marker, but SDS-PAGE of pET-MxMYB1 proteins uninduced by IPTG had not this band. These results will more provide the foundation for purifying and identifying objective protein.