银条根茎离体诱导

Inducement in Vitro of Rhizome of Stachys floridana Schuttl.ex Benth.

在无诱导剂的培养基上,低于4%的蔗糖不能刺激银条(StachysfloridanaSchuttl.exBenth.)产生根茎;而10%蔗糖的诱导效果在MB和MS培养基间无显著差异。葡萄糖(10%)比同浓度蔗糖能显著提高试管根茎数,但不引起总鲜样质量的增加。每个银条植株在含4%蔗糖的MS培养基附加8·0mg·L-16-BA和10mg·L-1SA中可诱导产生质量0·45g的根茎3·33个,显著优于其它组合。根茎经流式细胞仪检测没有发生倍性变化。

Sucrose less than 4% in medium could not Schuttl. ex Benth. on the medium without revulsant. There stimulate formation of rhizome for Stachysfloridana was no significant revulsive difference between MS medium and MB medium with 10% sucrose. Glucose could induce significantly more rhizome than sucrose at the same concentration level, but could not make the increasing of gross fresh weight of rhizome. Every plant of Stachys floridana Schuttl. ex Benth. would be induced to produce 3.33 pieces and 0. 45 g of rhizomes on MS medium containing 4% sucrose, 8.0 mg · L^-1 6-BA and 10 mg · L^-1 SA, which was significantly better than other combinations. The change of ploidy level was not observed in the induced rhizomes according to the result of determination by flow cytometry.