山葵试管苗快繁体系优化初探

Studies on Optimizing Production of Wasabi Plantlet

通过最优回归设计,利用6-BA、NAA和维生素C不同浓度配比对山葵试管苗增殖系数进行调查,结果表明:当6-BA、NAA、维生素C的浓度分别为1·2504、0和1·9668mg/L时,增殖系数可达到6·8380。壮苗比较适宜的生根培养基为1/2MS+NAA0·01mg/L或者1/2MS,生根率达90%以上。未生根的壮苗试管苗可直接移栽驯化,且生根率可达80%以上,能进一步降低种苗的生产成本。

The study showed that the concerntration of 6-BA, NAA and Vitamin C affected plantlet propogation coefficients of Wasabi through optimization regression analysis. As a result, the suitable combination was selected, the proliferation coefficient might increase to 6. 8380 when treated by MS medium with 6-BA 1. 2504 mg/L and Vitamin C 1. 9668 mg/L. In rooting culture, 1/2MS medium or 1/2MS with 0. 01mg/L NAA were suitable for root development and growth, and rooting frequency reached above 90%. In acclimatization, the results showed that strong plantlets without rooting were fit for culture out of door with around 80% rooting frequency, therefore, production cost of wasabi seedlings decreased greatly without rooting phases.