火百合子房薄层培养器官发生的细胞组织学研究

Cytohistological Study of Morphogenesis in Thin Cell Layer Culture of Ovary of Lilium ‘Star Gazer’

以火百合(Lilium‘StarGazer’)子房薄层组织为外植体,接种在附加BA2·0mg·L-1和NAA0·2mg·L-1的MS培养基上,诱导出愈伤组织及器官。经细胞组织学观察表明:细胞启动及愈伤组织产生于外植体近轴面和侧面的切口边缘及内方,继而形成瘤状、棒状及胚珠状的突起,之后从这些愈伤组织分化形成器官。火百合子房薄层培养中器官发生有3种方式:①在愈伤组织表面形成芽;②在愈伤组织内部或近表面形成根;③在同一块愈伤组织表面形成芽,内部形成根,再通过维管系统连成1个完整的植株原始体。

Thin cell layers from the ovary of Lilium ‘Star Gazer＇ were cultured as explants on MS medium supplemented with BA 2.0 mg · L^-1 and NAA 0. 2 mg · L^-1 to induce callus and organs. Historical study showed that callus initiated on the edges and the insides of the incisions of adaxial surface and flank of explants, then they formed strumae, club and ovule-like tubers, finally, they developed into organs. There are three types of organogenesis of Lilium ‘Star Gazer＇s ovary ： ①buds formed from the surface of callus ; ②roots formed from inside or near the surface of callus ; ③buds formed from the surface of callus, while roots formed from the inside of the callus, then they were connected by vascular systems to form whole primary plants.